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. 2015 Nov 19;7(3):366–376. doi: 10.1002/jcsm.12067

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© 2015 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of the Society of Sarcopenia, Cachexia and Wasting Disorders

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Transient receptor potential vanilloid 2 (TRPV2) expression pattern in cancer cachexia muscles. (A) Immunoblot analysis of TRPV2 and glyceraldehyde‐3‐phosphate‐dehydrogenase (GAPDH) in skeletal muscle homogenates from control, Lewis lung carcinoma (LLC), colon‐26, and mdx mice. The relative amount of TRPV2 was normalized to a GAPDH internal control. Data indicate the mean ± standard error (n = 3–4). *P < 0.05 vs. control. (B) Immunohistochemical analysis of TRPV2 and dystrophin in frozen cross sections of skeletal muscles. Double immunostaining was performed using rabbit anti‐TRPV2 and mouse anti‐dystrophin followed by fluorescein isothiocyanate‐conjugated or rhodamine‐conjugated secondary antibodies, respectively. Scale, 100 µm.