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. 2016 May 11;11(5):e0154398. doi: 10.1371/journal.pone.0154398

Fig 1. Na+ loading protocol.

Fig 1

Mice were subjected to 7 days of regular diet (0.26% NaCl, ~10 mg/d) followed by 5 days of high-salt diet (8% NaCl, ~300 mg/d). Days -3 to -1 served as an acclimation period. BP and HR measurements (23 per day) were obtained between 16:30 and 18:45. Those obtained during acclimation, the first 8 daily hemodynamic measurements and the outliers (as defined in Material and Methods) were excluded from further analyses. Chow and water intake, diuresis, fecal output and biochemical parameters were measured at different time points in Nalgene diuresis cages.