Table 1. Oligonucleotides used for qPCR studies.
| Genes | Sense | Primers |
|---|---|---|
| A) Aorta | ||
| ‒ Hprt | forward | TGCTCGAGATGTCATGAAGG |
| reverse | AATGTAATCCAGGTCAGC | |
| ‒ Slc12a2 (NKCC1) | forward | AGGTTCTCCAAACTCACGGCCTG |
| reverse | GACCAAGTCCAGCAGCCTGCATC | |
| B) Heart | ||
| ‒ Nppa (ANP) | forward & reverse | Cat. no.: QT00366170 |
| ‒ Nppb (BNP) | forward & reverse | Cat. no.: QT00183225 |
| ‒ Ppia (CycA) | forward & reverse | Cat. no.: QT00177394 |
| ‒ Col1a1 (proCol1a1) | forward & reverse | Cat. no.: QT00370622 |
| ‒ Col3a1 (proCol3a1) | forward & reverse | Cat. no.: QT01083537 |
| ‒ Myh6 (α-MHC) | forward & reverse | Cat. no.: QT00190267 |
| ‒ Myh7 (β-MHC) | forward & reverse | Cat. no.: QT00189504 |
Sequences are written 5’ to 3’. Oligonucleotides used for amplification of heart genes were purchased from Qiagen. ANP, atrial natriuretic peptide; BNP, B-type natriuretic factor; Cat. no., catalogue number; CycA, cyclophilin A; Hprt, hypoxanthine-guanine phosphoribosyltransferase.