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. 2016 May 12;6:25741. doi: 10.1038/srep25741

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Copyright © 2016, Macmillan Publishers Limited

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(a) MODELLER predicted that SP55 and GGG-SP55 would be exposed on the surface of the VLPs. The models were viewed with UCSF Chimera. N-core is orange; SP55 and GGG-SP55 are blue; and C-core is cyan. (b) GGG-SP55 was coupled to N-LPETGG-C VLPs with greater than 90% efficiency. (c) Conjugated product was separated by sucrose gradient density ultracentrifugation and analyzed by Tricine-SDS-PAGE. (d) NAGE assay was performed to monitor the sucrose gradient separation of reacted mixture. (e) TEM analysis of genetically inserted HBc-SP55, HBc-SP70, and sortagged N-LPETGGG-SP55-C and N-LPETGGG-SP70-C VLPs. White bar: 50 nm.