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. 2016 May 11;11(5):e0155104. doi: 10.1371/journal.pone.0155104

Fig 1. Workflow from HTS to pathway analysis of miRNA top hits.

Fig 1

(A) A549 cells were reverse-transfected with a library of 1,200 miRNA mimics at a final concentration of 20 nM. Non-targeting miRNA mimic controls and siRNAs (siNP) to countermeasure H7N9 replication were used as negative and positive controls, respectively. 48 h after miRNA transfection, the cells were infected with H7N9 at MOI of 0.01 for 24 h. 24 h after infection, the plates were fixed and analyzed by ELISA. 10 top hits from HTS ELISA were validated in virus replication studies. Top hit has-miR-664a-3p was then investigated for pathway analysis and downstream target effects. (B) A plot of normalized Z-score values calculated based on the ELISA results showing a wide range of virus replication modulation. All miRNAs with a Z-score < -1.5 were subjected to further validation.