Fig 7. miR664i reduces the replication of other influenza A strains.

(A-B) A549 cells were transfected with miR664i at 25 nM for 48 h. Cells were infected with A/Ca (H1N1) at MOI 0.1 and A/Phi at MOI 0.05 for 48 h. NTC; Non-transfected control (A) Cells were fixed with 10% formalin and stained for viral NP and counterstained with DAPI. Percentage of infected cells was measured with Cellomics ArrayScan and graphed. Data are from 3 replicate wells +/- SEM. *p<0.05. (B) Supernatants from transfected and infected A549 cells were titrated on MDCK cell. TCID₅₀ values were calculated and graphed. (3 replicate wells) ± SEM.　 *p<0.05.