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. 2016 May 12;6:25970. doi: 10.1038/srep25970

Figure 3. Effect CR on rhythms in clock protein levels.

Figure 3

Representative Western blots for clock proteins; BMAL1, CLOCK, CRY1, PER1 and PER2 assayed in the livers of mice (liver samples from three mice were pooled together at each time point) subjected to the following feeding regimens: AL – ad libitum, CR – 30% calorie restriction, TR –time-restricted feeding, F – fasting. Light and dark bars on the top of the figure represent light and dark phase of the day. ZT0 is the time when light is on and ZT12 is the time when light is off. Food for CR and TR groups was provided at ZT14, F group received food at ZT14 for two weeks and did not receive the food at the day of tissue harvesting.