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. 2016 May 12;6:25728. doi: 10.1038/srep25728

Figure 7. Relationship between retention time (tr) of plasma-irradiated HBS and its potency for evoking [Ca2+]i responses.

Figure 7

Single-cell analyses of the [Ca2+]i response evoked by plasma-irradiated HBS at varying tr (ti = 30 s). (a) Time course of changes in the average [Ca2+]i of 3T3-L1 cells stimulated with plasma-irradiated HBS at tr = 30 s (red line), 300 s (blue line), and 600 s (green line). The HBS containing 10% calf serum (CS) [10% CS/HBS] is used as positive control for [Ca2+]i increase. The mean values ± SE obtained from 23 cells are shown. The mean MAX[Ca2+]i ± SE were 0.50 ± 0.05 (tr = 30 s, p < 0.001 versus tr = 300 s and tr = 600 s), 0.20 ± 0.06 (tr = 300 s), and 0.14 ± 0.04 (tr = 600 s). Statistical analysis was performed with Steel-Dwass test for multiple comparisons. (b) Definition of the parameters MAX[Ca2+]i, tdelay, trise and tfall for each cell. Histograms showing the distributions of (c) MAX[Ca2+]i, (d) tdelay, and (e) trise for tr = 30, 300, and 600 s.