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. 2016 May 12;6:25720. doi: 10.1038/srep25720

Figure 5.

Figure 5

IgA antibody titers in serum (A), tracheal fluid (B), bile (C), and Harderian gland (D) of SPF chickens immunized with the PBS i.m., blank Ag@SiO2-NPs i.m., plasmid pVAX I-F (o) DNA i.m., pFDNA-Ag@SiO2-NPs i.m., and pFDNA-Ag@SiO2-NPs i.n. IgA antibody titers in these samples were detected with ELISA. Data are presented as the mean ± standard deviation (n = 3). “**” indicates statistically significant difference at P < 0.01.