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. 2016 May 12;6:25720. doi: 10.1038/srep25720

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Contents of IFN-γ (A) and IL-2 (B) of SPF chickens immunized with the PBS i.m., blank Ag@SiO₂-NPs i.m., plasmid pVAX I-F (o) DNA i.m., pFDNA-Ag@SiO₂-NPs i.m., and pFDNA-Ag@SiO₂-NPs i.n. Data are presented as the mean ± standard deviation (n = 3). “*” indicate statistically significant difference at P < 0.05; “**” indicate statistically significant difference at P < 0.01.