. Author manuscript; available in PMC: 2016 May 12.

Published in final edited form as: FASEB J. 2007 Jan 11;21(4):1145–1152. doi: 10.1096/fj.06-7439com

TABLE 1.

Binding constants for albumin: RAGE interaction

	K_d (µg/ml)	B_max (ng/well)	IC₅₀ (µg/ml)
Alb	n.d.	n.d.	n.d.
HOCl-Alb (25:1)	15.7 ± 1.2	10.5 ± 0.9	n.d.
HOCl-Alb (50:1)	14.6 ± 0.3	20.3 ± 1.6	29.0 ± 5.1
HOCl-Alb (100:1)	10.6 ± 0.6	42.8 ± 5.2	14.4 ± 3.5
MPO-Alb	16.1 ± 1.1	24.6 ± 1.7	20.1 ± 3.8
AGE-Alb	46.9 ± 4.6	52.6 ± 4.2	51.9 ± 15.2

K_d and B_max values for ¹²⁵I-soluble receptor for advanced glycation end products (RAGE) binding to microtiter wells coated with the indicated albumin preparations are listed. IC₅₀ values for ¹²⁵I-sRAGE binding inhibition to AGE-albumin (AGE-Alb)-coated microtiter wells by albumin (Alb), HOCl-Alb (at indicated oxidant:protein molar ratio), MPO-Alb (calculated HOCl:protein molar ratio of 50:1), and AGE-Alb are given. Calculations were performed by nonlinear regression analysis (GraphPad Prism). n.d. = non determined.