Figure 1.

Effects of kinase inhibitors on carbachol and bradykinin‐induced contraction in rat bronchioles. Measurement of isometric tension in freshly isolated rat bronchioles. CCh (A–D) or BK (E–H) was applied cumulatively (0.01–100μM) at 5 min intervals. Representative traces show typical cumulative contractile responses to CCh (A) and BK (E). Arrows indicate the points where the first concentration was applied. Two responses were performed in each bronchiole, the second after application of the Src inhibitor PP2 (30 μM, 10 min, B: CCh, n = 8 or F: BK, n = 11), the Rho‐kinase inhibitor Y27632 (10 μM, 10 min, C: CCh, n = 6 or G: BK, n = 4), the FAK inhibitor PF‐573228 (10 μM, 10 min, D: CCh, n = 8 or H: BK, n = 5) or no inhibitor (not shown). Measurements were taken at the end of each 5 min exposure and data fitted by nonlinear regression. Data expressed as a % of that induced by 80 mM KPSS (mean ± SEM); see main text or Supporting Information Fig. S4 for effects on PD₂ and max values.