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. 2015 Nov 23;1:15054. doi: 10.1038/cddiscovery.2015.54

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Copyright © 2015 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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EDPs prevent LPS-induced cell death in HL-1 cardiac cells. HL-1 cardiac cells were stimulated with LPS (1 μg/ml) in the presence of 19,20-EDP (1 μM), DHA (100 μM) and/or MSPPOH (50 μM) for 24 h. (a) Cell viability was scored by trypan blue exclusion assay. (b) Mitochondrial activity was evaluated by MTT assay. (c) Contractility of HL-1 cells was scored by counting the number of beats per minute in five different cell clusters. Values are represented as mean±S.E.M. N=3 independent experiments. *P<0.05 treatment versus vehicle control; ^# P<0.05 treatment group versus LPS or LPS/MSPPOH.