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. 2015 Nov 23;1:15054. doi: 10.1038/cddiscovery.2015.54

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Copyright © 2015 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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EDPs limit LPS-induced damage to mitochondrial ultrastructure. (a) HL-1 cardiac cells were stimulated with LPS (1 μg/ml) with or without 19,20-EDP (1 μM) where indicated for 24 h. After 24 h, the whole-cell lysates were harvested and then analyzed by western immunoblotting for the levels of essential mitochondrial proteins. Representative western blots and the results of quantification are demonstrated in (a–d). HL-1 cardiac cells were treated as indicated above. Representative electron micrograph (EM) images of HL-1 cells are presented on (e–h). Black arrowheads demonstrate individual mitochondrion. Scale bar, 500 μm in diameter. Values are represented as mean±S.E.M. N=3 independent experiments.