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. 2015 Nov 23;1:15054. doi: 10.1038/cddiscovery.2015.54

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Copyright © 2015 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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EDPs preserve mitochondrial function following LPS-induced cytotoxicity. HL-1 cardiac cells were stimulated with LPS (1 μg/ml) in the presence of 19,20-EDP (1 μM), DHA (100 μM) and/or MSPPOH (50 μM) for 24 h. (a) Cells were harvested and transferred into Clark-electrode-based chamber connected to Oxygraph at 30 °C. Rates of respiration were measured in saponin-permeabilized cells using 10 mm glutamate and 5 mM malate as substrates. ADP-stimulated respiration was measured after addition of 1 mM ADP. The rates of respiration are expressed as respiratory control ratio (RCR). (b) The intracellular ratio between ADP and ATP was measured by chemolumenescent assay and normalized per μg of protein. Values are represented as mean±S.E.M. N=3 independent experiments. *P<0.05 treatment versus vehicle control; ^# P<0.05 treatment group versus LPS or LPS/MSPPOH.