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. 2015 Nov 23;1:15054. doi: 10.1038/cddiscovery.2015.54

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Copyright © 2015 Cell Death Differentiation Association

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Inhibition of SIRT1 activity abolished 19,20-EDP protective effects against LPS-induced cytotoxicity. HL-1 cardiac cells were stimulated with LPS (1 μg/ml) in the presence of 19,20-EDP (1 μM) and EX-527 (1 μM) for 24 h. (a) Cell viability was scored by trypan blue exclusion assay. (b) Mitochondrial activity was measured by MTT assay. (c) The relative rates of mitobiogenesis were assessed using ELISA detecting simultaneous expression of SDH-A (nDNA-encoded protein) and COX-I (mtDNA-encoded protein) in each well of plated HL-1 cells. The ratio between COX-I and SDH-A expressions represents the relative rate of mitobiogenesis. (d) NF-κB DNA-binding activity in the whole-cell lysates was measured by ELISA. Values are represented as mean±S.E.M. N=3 independent experiments. *P<0.05 treatment versus vehicle control; ^# P<0.05 treatment group versus LPS.