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. Author manuscript; available in PMC: 2017 May 10.
Published in final edited form as: Cell Rep. 2016 Apr 28;15(6):1125–1133. doi: 10.1016/j.celrep.2016.04.014

Figure 3. PCP MTs in the germarium direct rotation.

Figure 3

(A) ImSAnE unrolling of basal surface of CLASP-depleted ovariole, showing regions 2b through st. 3 stained with phalloidin and anti-acTub. (B) Nematic order shows intact actin alignment when MTs are disrupted. (n≥5 for each stages, **p<0.01) (C, D) Control stage 9 follicle and mature eggs are elongated, while CLASP-RNAi stage 9 follicles and mature eggs are round. (E) Rotation speeds of st. 6-7 follicles, depleted at specified times using tj> CLASP RNAi tubGAL80ts, or treated with the MT polymerization inhibitor colchicine or the Arp2/3 inhibitor CK-666. (F) Quantitation of tj> CLASP RNAi tubGAL80ts egg shape.