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. Author manuscript; available in PMC: 2016 May 12.
Published in final edited form as: Biochem J. 2008 Apr 1;411(1):191–199. doi: 10.1042/BJ20071428

Figure 2. Prx IV is not secreted and remains ER associated.

Figure 2

A. HT1080 cells over-expressing Prx IV were pulsed with 35S-labelled methionine & cysteine for 30 min then chased for 5 hours with fresh medium. Cells and media were separated at the indicated times and radioactive Prx IV immunoprecipitated from each sample before being visualised by SDS-PAGE and autoradiography. B. Fluorescence microscopy of HT1080 cells following 5 hours incubation with 0.5 mM cycloheximide. Panels show a representative cell with nuclear staining (DAPI), immuno-staining of proteins containing the KDEL ER retrieval motif (anti-KDEL) and immuno-staining of Prx IV along with composite image.