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. Author manuscript; available in PMC: 2016 May 12.
Published in final edited form as: Biochem J. 2008 Apr 1;411(1):191–199. doi: 10.1042/BJ20071428

Figure 3. Stable alteration of Prx IV expression does not induce ER stress.

Figure 3

A. Western blot analysis of whole-cell lysates prepared from HT1080 cells and HT1080s engineered to stably overexpress (HT Prx IV) and under-express (shRNA1 and shRNA2) Prx IV. Prx IV expression levels (% Prx IV) relative to the parent cells are indicated ± standard deviation (n = 4). Anti-tubulin blot serves as a loading control. B. Agarose gel stained with ethidium bromide and imaged under UV light to visualise Pst I digests of XBP1 cDNA prepared from cell lines utilised in A. Negative control is prepared from the untreated HT1080s, positive control = HT1080s treated for 2 hours with 10 mM DTT. C. Western blot analysis of whole cell lysates to examine expression levels of BiP (with tubulin loading control). HT1080 lysate again serves as negative control and HT1080s treated for 12 hours with 10 µg/ml tunicamycin provide a positive control for UPR induction.