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. Author manuscript; available in PMC: 2016 May 12.
Published in final edited form as: Biochem J. 2008 Apr 1;411(1):191–199. doi: 10.1042/BJ20071428

Figure 6. Cysteine 51 is required to form a non-catalytic disulphide in Prx IV.

Figure 6

A. Linear representation of Prx IV indicating signal peptide (grey) and positions of cysteine residues. Peroxidatic (P) and resolving (R) cysteines are highlighted. B. Anti-Prx IV Western blots of HT1080 cell lysates following transient expression of cDNA encoding Prx IV native sequence (WT), or Prx IV with indicated cysteine residues mutated to alanine. Free thiols were alkylated with NEM prior to lysis.