Figure 5.

CYC1 expression is not essential for any other unit of OXPHOS. MDA-MB-231 and MDA-MB-435S cells were treated with control siRNA and CYC1 siRNA. The mRNA expression levels of the complex III subunits were identified via RT-PCR amplification in MDA-MB-231 cell (a) and MDA-MB-435S cell (b). Protein expression levels of mitochondrial complex III subunits were assessed via western blot (c). NDUFS1, SDHA, COX IV, and OSCP were, respectively, recognized as makers of mitochondrial complexes I, II, IV, and V. Protein expression levels of mitochondrial complexes I, II, IV, and V were measured by western blot (d).