Figure 4.

Distribution of RNA m⁵C residuals on mRNA and lncRNA. We divided all the Bismark reported C residuals into 3 groups; that is, 9514 residuals with methylation level higher than 1.65%, 8600 lower, and 406513 residuals cannot be determined with statistical significance. We can see that, for all 3 groups, there exists a strong 5′ bias on the mouse RNA BS-Seq data on both mRNA and lncRNA. The highly m⁵C methylated residuals are strongly enriched at 5′UTR of mRNA.