Table 2.
In vivo evaluation of transmission-blocking effects of anti-PSOPs immunization in direct mosquito feeding experiments.
| Exp. | Treatmenta | Median oocyst number (IQRb) | Mean oocyst number (SEMc) | % reductiond | Pe | Infected/Dissected (%)f | % reductiong | Ph |
|---|---|---|---|---|---|---|---|---|
| # 1 | Control | 62.5 (8.3-139.5) | 81.3 (14.42) | 27/28 (96.4) | ||||
| PSOP25 | 4.5 (0-39) | 30.6 (10.69) | 62.3 | 0.002 | 17/26 (65.4) | 31 | 0.004 | |
| PSOP7 | 17.0 (1.8-46) | 31.8 (6.858) | 60.8 | 0.009 | 22/28 (78.6) | 17.8 | 0.051 | |
| PSOP26 | 6.0 (0-60.5) | 32.5 (9.153) | 60.0 | 0.002 | 18/29 (62.1) | 34.3 | 0.001 | |
| # 2 | Control | 62.5 (4.5-146.5) | 94.6 (19.08) | 25/28 (89.3) | ||||
| PSOP25 | 7.5 (0-43.3) | 32.1 (10.64) | 66.0 | 0.009 | 18/26 (69.2) | 20.1 | 0.067 | |
| PSOP7 | 11.0 (1-49.8) | 30.1 (6.714) | 68.1 | 0.013 | 22/28 (78.6) | 10.7 | 0.234 | |
| PSOP26 | 2.0 (0-39.8) | 27.7 (8.134) | 70.7 | 0.002 | 18/30 (60) | 29.3 | 0.011 | |
| # 3 | Control | 49.0 (3.5-124.3) | 72.6 (15.14) | 25/28 (89.3) | ||||
| PSOP25 | 6.0 (0-42.3) | 25.5 (8.347) | 64.8 | 0.008 | 17/26 (65.4) | 23.9 | 0.036 | |
| PSOP7 | 7.0 (0.25-46.5) | 28.8 (7.571) | 60.3 | 0.031 | 21/28 (75) | 14.3 | 0.148 | |
| PSOP26 | 2.0 (0-44) | 24.9 (8.009) | 65.7 | 0.003 | 14/27 (51.9) | 37.4 | 0.002 |
In vivo TB activity assay was carried out using mice immunized with respective recombinant proteins in three experiments.
IQR, interquartile range.
SEM., standard error of arithmetic mean.
% reduction in oocyst intensity was calculated as (meancontrol - meanrPBANKA)/meancontrol × 100%.
P values from Mann–Whitney U test comparing the control and immunized groups.
Prevalence of infection was calculated as the number of mosquitoes with oocysts/total mosquitoes dissected.
% reduction in prevalence was calculated as % prevalence control – % prevalence immunization.
P values from Fisher's exact test for comparing the control and immunized groups.