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. 2015 Jun 24;40(13):2938–2947. doi: 10.1038/npp.2015.147

Figure 3.

Figure 3

Oxytocin (OXT) action on intracellular Ca2+ concentration ([Ca2+]i) is mediated via transient receptor potential vanilloid type-2 channel (TRPV2) channels in primary hypothalamic cells. (a and c) The TRPV channel blocker ruthenium red (10 μM; RR) has no influence on [Ca2+]i, but prevents the effects of 100 nM OXT on [Ca2+]i and oscillation amplitude and frequency (see Figure 2c). (b and d) The TRPC/TRPV2 channel blocker SKF96365 (100 μM) has no effect on [Ca2+]i, but additional application of OXT (100 nM) significantly decreases [Ca2+]i and stops the oscillations. Data represent mean+SEM. *P<0.05 (e) TRPV2 expression in the hypothalamic primary cell culture. +, positive control (hypothalamic tissue from adult rats); pcc, primary cell culture; −, negative control (without reverse transcription). (f) TRPV2 immunoreactivity in OXT-positive cells. Left: TRPV2 staining; middle: OXT staining; right panel: merged. White arrows show double-positive cells. Scale bar: 20 μm.