Fig. 4.

Time course of feather degradation by MBRL 575. a Feather weight loss and keratinase production as a function time. MBRL 575 was grown in CFM and measured the keratinase activity and feather weight loss at 12 h time interval. Each point represents the mean of three independent experiments. b Release of soluble peptides and amino acids by MBRL 575 at 12 h time intervals. Cell-free supernatant was used for the measurement of soluble peptides by the Folin’s method and amino acids by ninhydrin method. Each point represents the mean of three independent experiments