Table 1.

Effect of different factors on feather degradation by MBRL 575

Effect/treatment	Feather weight loss (%)	Keratinase activity (U/ml)	Soluble peptides (mg/g Feather)	Amino acids (mg/g feather)
Temperature (°C)
25	41 ± 3.6	80 ± 2	100 ± 2.5	109 ± 3.6
28	79 ± 3.6	268 ± 4.04	111 ± 3.05	123 ± 3.6
30	98 ± 0.4	305 ± 12	120 ± 5.5	158 ± 4
35	48 ± 2	95 ± 3.05	102 ± 6.1	108 ± 2
Feather concentration (w/v %)
0.5	100 ± 0.5	145 ± 7	161 ± 3	180 ± 6
1.0	98 ± 0.4	305 ± 12	120 ± 5.5	158 ± 4
2.0	85 ± 1	237 ± 11	110 ± 2.6	135 ± 2.5
3.0	76 ± 2	139 ± 7.7	98 ± 2	116 ± 3.05
5.0	61 ± 2.5	104 ± 6.02	80 ± 2.5	94.3 ± 4.04
7.0	53 ± 2.5	77 ± 7.5	81 ± 5.56	94 ± 3
10	45 ± 3	32 ± 4	81.3 ± 6.4	90 ± 2
Pre-treatment of feather
Non-autoclaved	46 ± 4	107 ± 8	58 ± 8.2	98 ± 2
One cycle autoclaved	98 ± 0.5	305 ± 12	120 ± 5.5	158 ± 4
Two cycle autoclaved	98 ± 2	310 ± 10	122 ± 4	162 ± 6
Three cycle autoclaved	99 ± 3	316 ± 7	126 ± 6.2	160 ± 8.2