Table 2.
Effect of carbon and nitrogen supplements on keratinase, soluble peptides and amino acid production
| Keratinase (U/ml) | Soluble peptides (mg/g feather) | Amino acids (mg/g feather) | |
|---|---|---|---|
| Carbon source (1 %, w/v) | |||
| Controla | 305 ± 12 | 120 ± 5.5 | 158 ± 4 |
| Glucose | 452 ± 6 | 136 ± 7 | 168 ± 14 |
| Na Citrate | 482 ± 16 | 150 ± 8.5 | 227 ± 18 |
| Corn starch | 418 ± 20 | 130 ± 4 | 176 ± 8 |
| Lactose | 280 ± 10 | 88 ± 6.2 | 102 ± 6.4 |
| Sucrose | 394 ± 14 | 129 ± 12 | 154 ± 2.4 |
| Nitrogen source (0.5 %, w/v) | |||
| Peptone | 350 ± 8 | 130 ± 6 | 176 ± 6 |
| Yeast extract | 338 ± 10 | 107 ± 16 | 130 ± 14 |
| Soybean meal | 536 ± 24 | 164 ± 10 | 263 ± 8 |
| Beef extract | 362 ± 6 | 125 ± 10.2 | 173 ± 12 |
| KNO3 | 209 ± 8 | 115 ± 8 | 54 ± 6 |
| NH4Cl | 17 ± 3 | 52 ± 4 | 87 ± 4 |
aControl represents CFM medium without any supplementary carbon or nitrogen source