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. 2016 Jan 28;5(5):892–902. doi: 10.1002/cam4.651

Figure 5.

Figure 5

Expression of miR‐143, PAI‐1 and MMP‐13 in human osteosarcoma tissue samples. (A–F) PAI‐1 and MMP‐13 immunostained primary tumors of patients. Scale bars, 50 μm. Central graph: The level of miR‐143 in 22 primary osteosarcoma specimens, including patients (A–F), was measured using real‐time reverse transcription (RT)‐PCR. Individual data points are the means of triplicate measurements from single RNA samples. The expression level of miR‐143 was normalized to that of miR‐103. Red symbols indicate lung metastasis‐positive (meta +) samples and blue symbols indicate lung metastasis‐negative (meta −) samples. The black bar indicates the mean value (0.58). PAI‐1, plasminogen activator inhibitor‐1; MMP‐13, matrix metalloproteinase‐13; PCR, polymerase chain reaction.