Skip to main content
. Author manuscript; available in PMC: 2016 May 12.
Published in final edited form as: Chem Res Toxicol. 2016 Feb 26;29(3):415–420. doi: 10.1021/acs.chemrestox.6b00009

Figure 2.

Figure 2

The effects of GE-1 and GE-2 on DNA cleavage mediated by human topoisomerase IIα. Double-stranded DNA cleavage levels in the presence of 0-1000 μM GE-1 (red) or GE-2 (blue) were calculated relative to a reaction that contained enzyme but no compound. A DNA control also is shown (DNA). Error bars represent standard deviations for at least three independent experiments. Ethidium bromide-stained agarose gels depicting DNA cleavage are shown (top). The mobility of negatively supercoiled DNA (form I; FI), nicked circular plasmid (form II; FII), and linear molecules (form III; FIII) are indicated. Gels are representative of five independent experiments.