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. Author manuscript; available in PMC: 2016 May 12.
Published in final edited form as: Chem Res Toxicol. 2016 Feb 26;29(3):415–420. doi: 10.1021/acs.chemrestox.6b00009

Figure 3.

Figure 3

Effects of GE-1 and GE-2 on DNA cleavage site utilization by human topoisomerase IIα. An autoradiogram of a 6% polyacrylamide gel is shown. A singly 32P-end-labeled linear 4332 bp fragment of pBR322 was used as the cleavage substrate. DNA cleavage reactions were carried out in the presence of 0-1000 μM GE-1 or GE-2, or 10 μM etoposide (as a positive control, Etop). A DNA control also is shown (DNA). Results are representative of three independent experiments.