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. Author manuscript; available in PMC: 2016 May 12.
Published in final edited form as: Chem Res Toxicol. 2016 Feb 26;29(3):415–420. doi: 10.1021/acs.chemrestox.6b00009

Figure 4.

Figure 4

DNA cleavage induced by GE-2 is reversible and protein-linked. Assay mixtures contained DNA and 250 μM GE-2 in the absence of enzyme (-TII), DNA in the presence of topoisomerase IIα in the absence of GE-2 (+TII), or reaction mixtures that contained DNA, enzyme, and 250 μM GE-2 that were stopped with SDS (SDS). To determine whether the reaction was reversible, EDTA was added prior to SDS (EDTA). To determine whether the cleaved DNA was protein-linked, proteinase K treatment was omitted (ProK). The mobility of negatively supercoiled DNA (form I; FI), nicked circular plasmid (form II; FII), and linear molecules (form III; FIII) are indicated. Error bars represent the standard deviation of three independent experiments. A gel that is representative of three independent experiments is shown (top).