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. 2016 Jan 13;34(2):268–276. doi: 10.1002/stem.2267

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© 2016 The Authors Stem Cells published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Methods of cancer stem cell characterization in human pituitary adenoma. Fresh tumor tissue can be dissociated and cancer stem cells (CSC) selected in vitro based on culture properties and marker expression. Assays to determine CSC potential are in vitro differentiation to determine their multipotent nature for appropriate tissue lineages, assays to determine possible resistance to cytotoxic compounds (characteristic of CSCs), and xenotransplantation into animal models where CSCs are expected to reinitiate tumor growth. Abbreviation: CSC, cancer stem cell.