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. 2016 May 11;9:103. doi: 10.1186/s13068-016-0517-y

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — qRT-PCR analysis of S. cerevisiae and C. acetobutylicum strains. Cycle threshold (Ct) values from three technical replicates were averaged. Fold differences were calculated using the reference gene ACT1 for S. cerevisiae strains the housekeeping gene CA_C0279 for C. acetobutylicum strains. a Results of qRT-PCR for genes related to ethanol and glycerol metabolism in S. cerevisiae. noxE NADH oxidase, ALD3 aldehyde dehydrogenase, ALD6 aldehyde dehydrogenase, ADH1 alcohol dehydrogenase, GPD1 glycerol-3-phosphate dehydrogenase, GUT1 glycerol kinase, TDH2 glyceraldehyde-3-phosphate dehydrogenase, HXT6 hexose transporter. b Results of qRT-PCR for genes related in ABE and acid metabolism in C. acetobutylicum. noxE NADH oxidase, Pta phosphotransacetylase, AskA acetate kinase, ctfA butyrate-acetoacetate CoA-transferase, Adc acetoacetate decarboxylase, Buk butyrate kinase, Ptb phosphate butyryltransferase, adhE bifunctional acetaldehyde-CoA/alcohol dehydrogenase, hydA hydrogen dehydrogenase