Table 2.
genes and primers for quantitative real-time PCR
| Gene ID | Gene name | Primer sequences |
|---|---|---|
| YFL039C | ACT1 (reference gene) | F: TGGATTCCGGTGATGGTGTT R: TGGCGTGAGGTAGAGAGAAACC |
| L196579 | noxE | F: TCAAAAATGGCGCAATCAAG R: CCGCGTAAACATCTGGATCA |
| YMR169C | ALD3 | F: TGGCGGCTCAGTATTGGAA R: CGCATTCTAGTGTGATATCCTTAAGG |
| YPL061W | ALD6 | F: GACAAAGTCAACGGTAGAACAATCA R: GGCTCTAAGGTGGTGAAGTTCATG |
| YOL086C | ADH1 | F: GAAGGTGCCGGTGTCGTT R: ACCGATCTTCCAGCCCTTAAC |
| YDL022W | GPD1 | F: TCAATTTTTGCCCCGTATCTG R: GATAGCTCTGACGTGTGAATCAACA |
| YHL032C | GUT1 | F: GCCCCAGCTCGTGAAACA R: GGGCTTTCCGCTGGTTTT |
| YJR009C | TDH2 | F: TCCAAGAAAGAGACCCAGCTAACT R: GGAGTCAATGGCGATGTCAA |
| YDR343C | HXT6 | F: CGCTGCTATTGCAGAGCAAAC R: CGAGTGGGAGGCTGAGTCA |
| CA_C0279 | Housekeeping gene | F: AGAAGTGGGAGCACCTGTAAAAA R: CGGTTCAATCTTTCCTTCAACTTT |
| L196579 | noxE | F: TCAAAAATGGCGCAATCAAG R: CCGCGTAAACATCTGGATCA |
| CA_C1742 | Pta | F: GAAATTCAGACCGGATCTTGCT R: GCCGCTACTTCACTATCTATTGCA |
| CA_C1743 | AskA | F: CAATGGATATAAGTGCAGAAGGTTCTA R: CTTTGGTATCCCTTGCAATCATTA |
| CA_P0163 | CtfA | F: CAACCCAGATACTGGCAAAAAAC R: TGCACGTATTCTTTCCACTAGAGTTC |
| CA_P0165 | Adc | F: ACGCTATGGCGCCACTTAAT R: TGCAAGAATGTGAGAGCTAGAAACA |
| CA_C3075 | Buk | F: GGCGGACTCTTAAAGCCAATAGTA R: GCATGCTGACCTTGAACTCCTA |
| CA_C3076 | Ptb | F: CAACACTTGATGCAGCAATGC R: GCTAAAGGTCCGTCAACTACACAA |
| CA_P0035 | adhE | F: AGGGAGCAAGCGGAGATTTAT R: TGCCGCATCCAAGAGTAAATG |
| CA_C0028 | hydA | F: GGAAAATGCGGAGTCTGTATGG R: TGGCAACACAAGCAGCTCTAA |