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. 2016 May 12;9:269. doi: 10.1186/s13104-016-2072-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Effect of quality filtering on average library insert size. Average insert sizes for libraries Mlu₅₀_IS2, Mlu₅₀_IS3 and Mlu₅₀_IS4 were plotted as a function of progressive stringency filtering. Most relaxed stringency corresponds to unfiltered libraries (raw). During most discriminative filtering reads are only passing quality control, if at least 90 % of their nucleotides had a Phred quality score ≥20 (90;20)