Table 2.
Sequencing library categories
| Library category | Fragmentation parameters | RB:BB ratios | Avg. insert size (raw data) (bps) | Sequenced genomes |
|---|---|---|---|---|
| TS | DF 5 %, PIP 175 W, C/B 200, Du 25 s | TruSeq® DNA PCR-free LPP (LS protocol, 550 bps) | 641 ± 28 | Bce, Efa, Pst, Mlu |
| IS1 | DF 10 %, PIP 175 W, C/B 200, Du 25 s | 2.0:1 + 3.8:2 | 686 ± 33 | Bce, Efa, Sen, Pst, Mlu |
| IS2 | DF 2 %, PIP 175 W, C/B 200, Du 30 s | 2.2:1 + 4.2:2 | 990 ± 79 | Efa, Sen, Mlu50 |
| IS3 | DF 2 %, PIP 175 W, C/B 200, Du 20 s | 2.3:1 + 4.4:2 | 1211 ± 78 | Bce, Pst, Mlu, Mlu50 |
| IS4 | DF 2 %, PIP 175 W, C/B 200, Du 10 s | 2.4:1 + 4.6:2 | 1297 | Mlu50 |
Settings for genomic DNA fragmentation and fragment size selection during library preparation. TS, original Illumina TruSeq® DNA PCR-free LLP (no modification)
IS1-IS4, categories for library average insert size, where IS1 < IS2 < IS3 < IS4
The term Mlu50 refers to the corresponding genome sequenced at 2 × 25 bps. Genomes listed without index were sequenced at 2 × 200 bps throughout
Bce B. cereus, Efa E. faecalis, Pst P. stutzeri, Mlu M. luteu, Sen S. enterica, RB Resuspension Buffer, BB Bead Buffer, DF Duty Factor, PIP Peak Incident Power, C/B Cycles per Burst, Du Duration