Table 2.
PCR primer information
| Gene | Forward primer sequence | Reverse primer sequence | Primer bank ID |
|---|---|---|---|
| Acox1 | TAACTTCCTCACTCGAAGCCA | AGTTCCATGACCCATCTCTGTC | 26333821a1 |
| Acsl1 | TGCCAGAGCTGATTGACATTC | GGCATACCAGAAGGTGGTGAG | 31560705a1 |
| Atg1 | AGATGAAAGCAAGATGTTGCCT | CCCTGTAGGTCAGCCATATTCTA | 27923915a1 |
| Atrogin‐1 | CAGCTTCGTGAGCGACCTC | GGCAGTCGAGAAGTCCAGTC | 13385848a1 |
| CD137 | CGTGCAGAACTCCTGTGATAAC | GTCCACCTATGCTGGAGAAGG | 20306992a1 |
| Cidea | TGACATTCATGGGATTGCAGAC | GGCCAGTTGTGATGACTAAGAC | 6680944a1 |
| CPT1α | CTCCGCCTGAGCCATGAAG | CACCAGTGATGATGCCATTCT | 27804309a1 |
| CPT1β | GCACACCAGGCAGTAGCTTT | CAGGAGTTGATTCCAGACAGGTA | 6753512a1 |
| Dio2 | AATTATGCCTCGGAGAAGACCG | GGCAGTTGCCTAGTGAAAGGT | 6753638a1 |
| Gapdh | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA | 6679937a1 |
| Glut1 | GCCTGACCTTCGGATATGAGC | TGCCATAGCAGTCAATGAGGA | 18485498a1 |
| Glut4 | GTGACTGGAACACTGGTCCTA | CCAGCCACGTTGCATTGTAG | 6678015a1 |
| Hsl | CCAGCCTGAGGGCTTACTG | CTCCATTGACTGTGACATCTCG | 26325924a1 |
| IGF‐I | CTGGACCAGAGACCCTTTGC | GGACGGGGACTTCTGAGTCTT | 6754308a1 |
| MyoD | CCACTCCGGGACATAGACTTG | AAAAGCGCAGGTCTGGTGAG | 6996932a1 |
| Myogenin | GAGACATCCCCCTATTTCTACCA | GCTCAGTCCGCTCATAGCC | 13654247a1 |
| Myostatin | AGTGGATCTAAATGAGGGCAGT | GTTTCCAGGCGCAGCTTAC | 6754752a1 |
| MuRF‐1 | GTGTGAGGTGCCTACTTGCTC | GCTCAGTCTTCTGTCCTTGGA | 21523717a1 |
| Pax‐3 | CCGGGGCAGAATTACCCAC | GCCGTTGATAAATACTCCTCCG | 26377023a1 |
| Pax‐7 | TCTCCAAGATTCTGTGCCGAT | CGGGGTTCTCTCTCTTATACTCC | 34328055a1 |
| Pgc1α | TATGGAGTGACATAGAGTGTGCT | CAGGAGTTGATTCCAGACAGGTA | 6679433a1 |
| Pgc1β | TCCTGTAAAAGCCCGGAGTAT | GCTCTGGTAGGGGCAGTGA | 18875426a1 |
| Pparα | AGAGCCCCATCTGTCCTCTC | ACTGGTAGTCTGCAAAACCAAA | 31543500a1 |
| Pparδ | CAAGTGGGGTCAGTCATGGAA | GCTGGAAGGAAGCGTGTGTT | 403943a1 |
| Prdm16 | CCCCACATTCCGCTGTGAT | CTCGCAATCCTTGCACTCA | 124107622c3 |
| Tbx1 | CTGTGGGACGAGTTCAATCAG | TTGTCATCTACGGGCACAAAG | 22094109a1 |
| Tmem26 | TTCCTGTTGCATTCCCTGGTC | GCCGGAGAAAGCCATTTGT | 29244332a1 |
Appropriate primer sequence was obtained from http://pga.mgh.harvard.edu/primerbank/