Transcript levels of the MAMP-induced genes NHL10, PROPEP2 and PROPEP3 are reduced in agb1 mutants after flg22 treatment. Two-week old agb1-2 (A, C, E) and agb1-9 (B, D, F) seedlings were treated with 1 µM flg22 and sampled at the indicated time points after flg22 application. Transcript levels were determined by quantitative RT-PCR. (A, B)
NHL10, (C, D)
PROPEP2 and (E, F)PROPEP3 gene expression was normalized to the transcript levels of the reference gene At4g26420 and is presented relative to the transcript abundance of non-treated Col-0 wild-type at time point 0 minutes. A representative data set with mean ± standard deviation of three technical replicates per genotype and time point is shown. The experiment was performed three times with similar results.