Table 1.
Arabidopsis cyclic nucleotide binding protein candidates
| LEAF cAMP | LEAF cGMP | CALLUS cAMP | CALLUS cGMP | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Accession | Description | 2-AHA-cAMP-agarose | 8-AEA-cAMP-agarose | 2-[Biotin]-AHA-cAMP | cAMP antibody | 2-AH-cGMP-agarose | 8-AET-cGMP-agarose | 2-[Biotin]-AH-cGMP | cGMP antibody | 2-AHA-cAMP-agarose | 8-AEA-cAMP-agarose | 2-[Biotin]-AHA-cAMP | cAMP antibody | 2-AH-cGMP-agarose | 8-AET-cGMP-agarose | 2-[Biotin]-AH-cGMP | cGMP antibody |
| AT3G13920 | eukaryotic translation initiation factor 4A1 | B | B | B | B | B | B | B | B | B | B | B | |||||
| AT3G12780 | phosphoglycerate kinase 1 | B | B | B | B | B | B | B | B | ||||||||
| AT1G42970 | glyceraldehyde-3-phosphate dehydrogenase B subunit | B | B | B | 2, 3, B | B | B | B | B | ||||||||
| AT4G20360 | RAB GTPase homolog E1B | B | B | B | 2, 3, B | B | B | B | B | ||||||||
| AT3G60750 | Transketolase | B | B | B | B | B | B | B | |||||||||
| AT5G50920 | CLPC homologue 1 | B | B | B | B | B | B | B | |||||||||
| AT4G37930 | serine transhydroxymethyltransferase 1 | 2, B | B | 1, B | B | B | B | 2, 3, B | |||||||||
| AT3G14420 | Glycolate oxidase 1 | B | B | B | B | ||||||||||||
| AT4G09650 | ATP synthase delta subunit | B | B | B | B | ||||||||||||
| AT1G09340 | chloroplast stem-loop binding protein of 41 kDA | 1 | B | B | 2, B | ||||||||||||
| AT1G56330 AT3G62560 AT4G02080 | secretion-associated RAS 1B | B | B | B | |||||||||||||
| AT4G27440 | protochlorophyllide oxidoreductase B | B | B | B | |||||||||||||
| AT3G01500 | carbonic anhydrase 1 | B | B | ||||||||||||||
The fraction in which the protein was identified is indicated with numbers 1–6 being the elution fractions (1 denotes the first and least stringent elution and 6 the final and most stringent elution) while B indicates that the protein was found in the bead fraction. For the cAMP baits the elutions were 1) 100 mM GDP; 2) 100 mM AMP; 3) 10 mM cGMP; 4) 100 mM cGMP; 5) 10 mM cAMP and 6) 100 mM cAMP. For the cGMP baits the elutions were 1) 100 mM ADP; 2) 100 mM GMP; 3) 10 mM cAMP; 4) 100 mM cAMP; 5) 10 mM cGMP and 6) 100 mM cGMP