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. 2016 May 12;12:27. doi: 10.1186/s13007-016-0127-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Confocal time lapse imaging during LRP to shoot conversion. a–d Time-lapse imaging showing PLT2-vYFP expression during the conversion of LRP to shoot. Note the down-regulation of PLT2-vYFP during the development of shoot meristem. e–h Dynamic expression pattern of pSCR::H2B-vYFP during the conversion of root to shoot. i–l Live imaging showing STM-vYFP expression during the shoot conversion. l Note the appearance of STM-vYFP in the nascent shoot meristem. m–p Spatiotemporal expression pattern of pWUS::erCFP. p Note the rapid upregulation of pWUS::erCFP upon cytokinin treatment. Arrowhead in (d, h, l, p) marks leaf primordium. Red signal is propidium iodide stain in (a, b, e, f, i, j, m, n) and FM4-64 stain in the remaining. Scale bar 50 µm