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Details of the settings for commonly used fluorescent signals
| Signal | Excitation wavelength (nm) | Emission wavelength range (nm) |
|---|---|---|
| CFP | 458 | 465–515 |
| GFP | 488 | 495–530 |
| YFP/VENUS | 514 | 520–545 |
| Propidium iodide | 514/561 | 585–630 |
| FM4-64 | 514/561 | 620–750 |
| Chlorophyll autofluorescence | 488/514/561 | 620–750 |
