Table 2. R2 values for regression analysis between microarray and q-PCRa.
Geneb | Probe 1c | Probe 2c | Maximum expression in datasetd | R2 | ||
---|---|---|---|---|---|---|
Probe 1 | Probe 2 | Probe averagee | ||||
HLA-DRA | ILMN_1689655 | ILMN_2157441 | 15.03 | 14.61 | 14.82 | 0.925f |
TAP1 | ILMN_1751079 | 14.78 | 0.759 | |||
COL1A1 | ILMN_1701308 | 14.75 | 0.948 | |||
CD14 | ILMN_1740015 | ILMN_2396444 | 13.20 | 14.80 | 14.00 | 0.732 |
ELANE | ILMN_1706635 | 13.87 | 0.783 | |||
CD34 | ILMN_1732799 | ILMN_2341229 | 13.59 | 13.54 | 13.57 | 0.944 |
CD19 | ILMN_1782704 | 13.49 | 0.985 | |||
PRTN3 | ILMN_1753584 | 13.16 | 0.867 | |||
CD33 | ILMN_1747622 | 11.66 | 0.834 | |||
KRT8 | ILMN_1753584 | 11.55 | 0.974 | |||
P2RX5 | ILMN_1677793 | 11.09 | 0.679 | |||
MOB3A | ILMN_1721344 | 10.88 | 0.326 | |||
POLE | ILMN_1728199 | 10.76 | 0.409 | |||
C19orf48 | ILMN_1759184 | ILMN_2383484 | 11.34 | 10.11 | 10.73 | 0.266 |
HMHA1 | ILMN_1811392 | 10.11 | 0.779 | |||
CLYBL | ILMN_1663538 | 9.30 | 0.487 | |||
ROR1 | ILMN_1655904 | 9.20 | 0.938 | |||
COIL | ILMN_1688034 | 8.65 | -0.001 | |||
MS4A1g | ILMN_1776939 | 8.62 | 0.320 | |||
PFAS | ILMN_1755862 | 8.61 | 0.216 | |||
PTPRCh | ILMN_2340217 | ILMN_1653652 | 8.07 | 8.78 | 8.43 | 0.347 |
APOBEC3B | ILMN_2219466 | 8.23 | 0.652 | |||
TTK | ILMN_1788166 | 8.09 | 0.545 | |||
WT1 | ILMN_1802174 | 8.00 | 0.856 |
a R2 values for individual genes were calculated from the regression model.
b Official gene symbols are depicted for genes as included in the q-PCR validation set.
c Illumina probe IDs as measured in the microarray database.
d Maximum probe fluorescence as measured by microarray gene expression analysis in the sample set for q-PCR validation.
e Average of maximum probe fluorescence is given for genes with more than 1 probe.
f R2 >0.667 are bold.
g MS4A1 is also known as CD20.
h PTPRC is also known as CD45.