Fig. 4.

Schlafen 3 reduction did not modulate intestinal epithelial proliferation induced by strain or EGF. A: IEC-6 cells transiently transfected with siRNA targeted to Schlafen 3 or with a nontargeting NT1 sequence were maintained under static conditions or cyclic strain for 24 h before crystal violet staining. Strain stimulated proliferation in both NT1-treated cells and cells in which Schlafen 3 was reduced (n = 3, *P < 0.05). IEC-6 cell monolayers exposed to cyclic strain for 24 h display significantly increased cell numbers after strain initiation compared with static control cell monolayers at the same time point, as assessed by a colorimetric assay using crystal violet absorbance assay over the linear range of the assay, with interpolation against a standard curve. However, reducing Schlafen 3 by siRNA could not block this strain effect. B: incubation IEC-6 cells with 10 pM EGF for 24 h resulted in a 1.68 ± 0.02-fold (n = 3, *P < 0.05) increase in proliferation. However, reduction of Schlafen 3 by transient transfection with siRNA targeted to Schlafen 3 also did not block EGF-induced IEC-6 proliferation compared with static control.