Fig. 5.

Cyclic strain (24 h) induction of Schlafen 3 protein was blocked by 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2; 10 μM) (A), SB203580 (SB) (10 μM) (B), or LY294002 (LY) (20 μM) (C), which block Src, p38, and phosphatidyl inositol (PI3)-K, but not by PD98059 (20 μM) (PD) (D), which blocks ERK (n = 4, *P < 0.05). p-FAK, phosphorylated FAK; t-FAK, total FAK. Each inhibitor significantly blocked basal as well as strain-stimulated phosphorylation or accumulation of the specific downstream target of each kinase (n = 3, lower two blots for each, *P < 0.05). E: Strain stimulation of Schlafen 3 expression was prevented by Src blockade with PP2 (10 μM) vs. actin mRNA (n = 7, *P < 0.01). FAK, focal adhesion kinase.