Table 1. Summary statistics for the CAOS analyses of the problematic groups at the species level.
Each of the investigated species showed intraspecific variation above 2%, suggesting that DNA barcoding may be hampered by the lack of a distinct barcoding gap. However, each of the different lineages within these species groups possesses diagnostic characters that can aid in the future identification of the lineages (no diagnostic characters where found when comparing the sequences to the entire pool of nemertean taxa; see text for discussion).
| Taxon | Number of sequences analyzed | Global, private nucleotides exist * | State: position † | Local, private nucleotides exist for any clade (for each clade) ‡ | Clade (state: position) § |
|---|---|---|---|---|---|
| Oerstedia dorsalis | 110 | No | N/A | Yes (Yes) | 1 (A:66, A:396); 2 (T:243, A:300); 3 (C:57, G:117); 4 (A:54, G:102, A:342, G:504); 5 (G:126, G:291, C:312); 6 (A:36, C:150, G:276, C:474, G:501); 7 (A:12, C:30, A:48, T:117, C:124, T:126, C:193, C:195, T:209, A:261, C:274, T:276, T:282, A:483, G:495); 8 (G:16, A:39, G:87, G:93, G:96, G:147, A:150, C:159, C:186, T:190, A:192, G:213, C:225, A:234, C:243, G:249, G:261, G:288, G:303, A:306, C:309, C:369, C:396, A:456, C:469, T:471, T:475, A:477, T:484, A:486, C:508) |
| Cerebratulus marginatus | 7 | No | N/A | Yes (Yes) | 1 (C:12, G:15, A:27, C:33, A:60, G:72, A:75, C:78, T:111, A:156, A:180, A:192, C:198, A:201, A:210, C:217, A:234, A:237, A:238, G:243, T:261, A:276, A:280, T:303, T:318, C:344, T:363, C:426, A:429, C:435, A:438, A:444, T:477, A:483, T:492, A:507); 2 (A:12, T:21, C:39, A:54, G:60, C:85, T:87, A:96, G:111, T:126, C:133, G:135, C:147, C:162, G:201, C:231, C:243, G:246, G:252, A:261, G:273, T:277, G:306, G:348, A:360, T:396, C:408, A:441, C:466, T:468, A:471, A:495, G:507, G:513); 3 (G:3, T:6, C:22, C:60, C:69, G:87, G:99, A:11, C:114, G:117, T:144, G:147, G:171, C:193, T:195, T:201, C:205, G:207, C:210, G:228, T:234, A:243, T:245, C:249, A:252, T:255, G:258, C:279, C:282, C:294, A:306, G:309, A:315, C:318, C:355, G:363, A:387, A:426, T:435, C:460, T:462, C:474, G:482, T:483, C:508, T:510); 4 (C:15, A:21, A:24, T:27, C:30, G:36, A:39, T:60, G:66, A:72, G:90, T:96, T:99, C:111, T:117, G:126, G:132, G:159, A:168, T:190, G:192, C:198, C:201, A:219, A:220, T:221, A:225, A:228, A:231, T:243, T:249, C:274, T:276, T:280, C:281, A:282, A:286, G:330, G:342, A:345, C:372, A:382, T:384, G:387, A:399, T:400, T:401, T:405, T:406, A:408, G:429, G:448, G:450, G:474, T:475, C:484, T:486, A:492, C:498, C:501) |
| Parborlasia corrugatus | 41 | No | N/A | Yes (Yes | 1 (G:9, A:24, T:27, T:54, C:57, G:60, T:81, C:85, G:111, G:117, T:120, A:123, T:124, G:126, T:150, C:153, A:168, C:174, A:198, T:210, G:231, G:270, T:279, C:294, G:303, A:315, T:328, A:330, C:369, T:381, A:411, T:426, G:510); 2 (A:9, T:15, G:24, C:27, T:39, C:54, A:60, C:81, A:111, A:117, G:123, C:124, T:126. G:150, T:153, T:168, T:174, G:198, C:210, T:231, A:270, C:279, T:294, T:303, G:315, C:328, T:330, T:369, G:381, G:411, G:436, A:510) |
| Tetrastemma melanocephalum | 6 | No | N/A | Yes (Yes) | 1 (A:21, T:22, A:24, A:36, A:48, G:54, A:96, A:126, C:132, G:141, C:150, G:156, T:190, A:192, G:195, C:198, G:207, C:209, G:213, T:214, G:216, G:225, T:228, G:231, T:234, A:240, A:243, G:255, T:261, A:264, G:270, T:274, G:276, G:295, A:300, A:303, G:309, G:315, T:328, A:330, C:333, G:360, T:405, A:429, T:441, T:453, A:456, C:460, T:469, A:471, C:475, T:480, T:501, A:510); 2 (G:6, A:12, A:87, C:90, T:108, G:132, G:144, G:168, A:183, G:208, G:237, G:243, G:273, T:282, C:291, A:363, G:381, G:396, G:405, A:420, G:447, G:462, C:466, T:468, A:477); 3 (G:15, A:18, C:37, T:39, C:45, T:72, A:108, A:111, G:123, T:132, A:144, G:159, T:177, A:189, G:201, C:210, C:217, T:243, A:258, C:270, C:294, A:399, A:405, G:408, G:423, A:438, A:457, A:504, T:513) |
| Tetrastemma robertianae | 6 | No | N/A | Yes (Yes) | 1 (C:1, T:3, T:6, G:18, T:48, A:60, T:72, G:87, G:90, T:99, A:102, G:111, G:117, G:132, A:141, C:159, T:168, A:171, G:189, C:193, T:195, T:201, T:210, C:217, T:219, A:234, A:237, A:240, T:243, T:255, A:258, A:261, T:264, T:270, A:273, T:279, A:282, A:291, A:300, A:309, A:318, T:327, G:342, A:345, C:348, T:363, G:385, A:396, A:399, C:405, G:408, G:420, G:423, G:435, A:438, A:444, A:447, A:453, A:462, C:463, A:468, C:469, T:471, G:483, G:492, A:513); 2 (T:1, G:3, A:6, T:18, A:48, T:60, A:72, A:87, T:90, A:99, G:102, T:111, A:117, T:132, G:141, T:159, G:168, G:171, A:189, T:193, G:201, A:210, T:217, A:219, G:234, G:237, T:240, G:243, G:255, G:258, T:261, A:264, C:270, T:273, A:279, T:282, T:291, G:300, T:309, T:318, A:327, T:342, T:345, A:348, A:363, A:385, G:396, G:399, A:408, A:423, T:435, G:438, T:444, G:447, T:453, G:462, T:463, G:468, T:469, A:471, A:483, T:492, G:513) |
* Result based on comparison of the entire pool of sequences for the taxon listed versus all sequences from the 915-taxon dataset.
† Relative position of the nucleotides based on the alignment used in the present study.
‡ Result based on comparison of each clade found in the NJ analysis versus the remainder of sequences with identical taxonomic labels.
§ Clade number refers to the numbers in the NJ tree (S1 Fig).