Table 1. Primer sequences used for Real-time PCR assay.
Name 1 | Sequence (5’→3’) 2 | Genbank 3 |
---|---|---|
β-Actin | CTGTCCCTGTATGCCTCTG | NM_007393.3 |
ATGTCACGCACGATTTCC | ||
Nrf2 | CAGTGCTCCTATGCGTGAA | NM_010902.3 |
GCGGCTTGAATGTTTGTC | ||
HO-1 | ACAGATGGCGTCACTTCG | NM_010442.2 |
TGAGGACCCACTGGAGGA | ||
MnSOD | CCGAGGAGAAGTACCACGAG | NM_013671.3 |
GCTTGATAGCCTCCAGCAAC | ||
GPx1 | AGTATGTGTGCTGCTCGGCTCT | NM_008160.6 |
CCAGTAATCACCAAGCCAATGC | ||
NQO1 | CTTTAGGGTCGTCTTGGC | NM_008706.5 |
CAATCAGGGCTCTTCTCG | ||
SIRT1 | TGCAGACGTGGTAATGTCCAAAC | NM_019812.2 |
ACATCTTGGCAGTATTTGTGGTGAA |
1 Huclear factor erythroid 2-related factor 2 (Nrf2); Heme oxygenase 1 (HO-1); Manganese superoxide dismutase (MnSOD); Glutathione peroxidase 1 (Gpx1); NAD(P)H quinone oxidoreductase 1 (NQO1); Sirtuin 1 (Sirt1).
2 Shown as forward primer followed by reverse primer.
3 GenBank Accession Number.