Table 1. The kinetic parameters, Km and Vm, measured at 5μM and 100μM PLP for ClACS7, W364, K264F395 and F395 protein isoforms.
Enzyme | 5 μM PLP | 100 μM PLP | ||
---|---|---|---|---|
Km (μM) | Vm (nmol.min-1.mg-1) | Km (μM) | Vm (nmol.min-1.mg-1) | |
ClACS7 | 4.6 ± 1.8 | 950 ± 80 | 5 ± 2 | 1000 ± 50 |
W364 | 4.5 ± 1.8 | 240 ± 20 | 5 ± 3 | 900 ± 80 |
K264F395 | nd | ≤ 10 | nd | ≤ 10 |
F395 | 3.2 ± 0.7 | 390 ± 10 | 5 ± 3 | 960 ± 100 |