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. 2016 May 12;12(5):e1006035. doi: 10.1371/journal.pgen.1006035

Fig 2. Strategies for inducing expression of scFv in PV cells or in CSF.

Fig 2

A) Co-staining for Myc and PV in layers II-IV of the binocular zone of the visual cortex (V1b) of P30 PV::Cre, PV::Cre;scFvPax6tg/o and PV::Cre;scFvOtx2tg/o mice (scale bar: 100μm). The enlarged view shows the immunofluorescence overlap. B) Western blot of cerebrospinal fluid (CSF) protein extracts from scFvOtx2tg/o and scFvPax6tg/o adult mice injected (+Cre) or not (ctrl) with Cre-TAT protein in lateral ventricles. Anti-Otx2 and anti-Pax6 scFvs (revealed with anti-Myc antibody) are secreted in the CSF upon choroid plexus recombination.