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. 2016 May 12;12(5):e1006035. doi: 10.1371/journal.pgen.1006035

Fig 4. Anti-Otx2 scFv in adult CSF activates plasticity genes.

Fig 4

A) Immunostaining for Otx2, PV, WFA, CR and GABA in V1b layers II-IV of adult scFvPax6tg/o and scFvOtx2tg/o mice, 15 days after intracerebroventricular (icv) injection of Cre-TAT protein (scale bar: 100μm). B) Quantification of number of Otx2-, PV-, WFA, CR- and GABA-positive cells in V1b layers II-IV of adult scFvOtx2tg/o and scFvPax6tg/o mice, 15 days after icv injection of Cre-TAT (in percentage of scFvPax6tg/o mice, dashed line indicates scFvPax6tg/o levels). Absolute numbers are provided in S2 Fig. C) Quantification of Arc, Fos, Nr4a1, Egr4 and HPRT mRNA content (relative to GAPDH) in V1 layer IV extracts from adult scFvOtx2tg/o and scFvPax6tg/o mice, 15 d after icv injection of Cre-TAT. (t-tests; 3–12 mice per group; *p<0.05, **p<0.01, ***p<0.001; error bars indicate SEM)