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. 2016 May 12;11(5):e0155668. doi: 10.1371/journal.pone.0155668

Fig 1. Identification of cervical DC subsets from (ecto) cervical explant cultures.

Fig 1

(A) Plots showing the exclusion of dead cells (VIVID+), doublets, neutrophils/epithelial cells (CD66a/c/e+) and monocytes (CD66a/c/e− CD14+). (B) Dendritic cell subsets were identified as mDCs (CD66a/c/e− CD14− CD11c+); pDCs (CD66a/c/e− CD14− CD11c− CD123+); and LCs (CD66a/c/e− CD14− CD11c− CD123− CD1a+); and frequencies of cervical tissue migrating DCs determined, following different stimulation conditions. (C) Histogram showing up-regulation of HLA-DR expression by cervical explant migrating mDCs. Activation of pDCs and LCs was determined in a similar way as mDCs and Median Fluorescent Intensities (MFI) calculated.